Table of Contents

Laboratory Title Page
Laboratory Signature Page
Laboratory QA Statement
Laboratory Compliance Page
SUMMARY
INTRODUCTION
   PURPOSE
   CHARACTERIZATION OF TEST AND CONTROL ARTICLES
MATERIALS AND METHODS
   TEST SYSTEM
   METABOLIC ACTIVATION SYSTEM
   SOLUBILITY TEST
   PRELIMINARY TOXICITY ASSAY
   CHROMOSOME ABERRATION ASSAY
   COLLECTION OF METAPHASE CELLS
   SLIDE PREPARATION
   EVALUATION OF METAPHASE CELLS
   EVALUATION OF TEST RESULTS
   CRITERIA FOR A VALID TEST
   ARCHIVES
RESULTS AND DISCUSSION
   SOLUBILITY TEST
   PRELIMINARY TOXICITY ASSAY
   CHROMOSOME ABERRATION ASSAY
CONCLUSION
REFERENCES
LIST OF TABLES
1 Preliminary Toxicity Test in the Absence of Exogenous
    Metabolic Activation
2 Preliminary Toxicity Test in the Presence of Exogenous
    Metabolic Activation
3 Preliminary Toxicity Test in the Absence of Exogenous
    Metabolic Activation
4 Preliminary Toxicity Test in the Presence of Exogenous
    Metabolic Activation
5 Concurrent Toxicity Test in the Absence of Exogenous
    Metabolic Activation
6 Cytogenetic Analysis of Cells Treated (Absence of
    Exogenous Metabolic Activation)
7 Concurrent Toxicity Test in the Presence of Exogenous
    Metabolic Activation
8 Cytogenetic Analysis of Cells Treated (Presence of
    Exogenous Metabolic Activation)
9 Summary
Appendix A: Historical Control Data
Appendix B: Study Protocol

Abstract

Evaluates the clastogenic potential of tertiary amyl methyl ether (TAME) using Chinese hamster ovary (CHO) cells compared to the solvent control group. Based on the findings of this study, TAME was concluded to be positive for the induction of structural chromosome aberrations in Chinese hamster ovary (CHO) cells.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	American Petroleum Institute
Pages
ISBN