Table of Contents

1 -  AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
1 -  PREFACE
2 -  METHOD
2 -  1 SCOPE
2 -  2 REFERENCED DOCUMENTS
2 -  3 DEFINITIONS
2 -  3.1 Coliforms
2 -  3.2 Thermotolerant coliforms
2 -  3.3 Escherichia coli (E. coli)
2 -  4 PRINCIPLE
3 -  5 CULTURE MEDIA AND REAGENTS (See Appendix B)
3 -  6 APPARATUS
3 -  7 REFERENCE CULTURES
3 -  7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
3 -  7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
3 -  7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
3 -  7.4 Use of reference cultures
4 -  8 SAMPLES
4 -  8.1 Laboratory samples
4 -  8.2 Dilutions
4 -  9 PROCEDURE
4 -  9.1 Presumptive test
5 -  9.2 Confirmatory tests
5 -  9.2.1 Coliforms
5 -  9.2.2 E. coli
6 -  9.2.3 Thermotolerant coliforms
6 -  10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
6 -  11 TEST REPORT
6 -  12 BIBLIOGRAPHY
7 -  APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS-MOST PROBABLE NUMBER
8 -  APPENDIX B - CULTURE MEDIA AND REAGENTS
8 -  B1 SCOPE
8 -  B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
10 -  B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
10 -  B4 NUTRIENT AGAR
11 -  B5 MACCONKEY AGAR
11 -  B6 OXIDASE REAGENT (KOVACS’)
11 -  B7 EC-MUG BROTH
12 -  APPENDIX C - PERFORMANCE OF KOVACS’ OXIDASE TEST

Abstract

Sets out a method, using a multiple tube dilution technique,
for determining the most probable number (MPN) of coliforms,
Escherichia coli (E. coli) and thermotolerant coliforms in
water.

RECONFIRMATION NOTICE
Technical Committee FT-020 has reviewed the content of this publication and in accordance with Standards Australia procedures for reconfirmation, it has been determined that the publication is still valid and does not require change.
Certain documents referenced in the publication may have been amended since the original date of publication. Users are advised to ensure that they are using the latest versions of such documents as appropriate, unless advised otherwise in this Reconfirmation Notice.
Approved for reconfirmation in accordance with Standards Australia procedures for reconfirmation on 16 August 2017.
Approved for reconfirmation in New Zealand on behalf of the New Zealand Standards Approval Board on 26 June 2018.
The following are represented on Technical Committee FT-020:
ACT Health
Australian Society for Microbiology Incorporated
Australian Water Association
Chemistry Australia
National Association of Testing Authorities Australia
National Measurement Institute
NSW Health
PathWest Laboratory Medicine WA
Queensland Health
Queensland University of Technology
The University of Melbourne
Victorian Water Industry Association
Water NSW
Water Services Association of Australia

Scope

This Standard sets out a method, using a multiple tube dilution technique, for determining the most probable number (MPN) of coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water. It is suitable for waters with high background flora and/or turbidity.
The method is suitable for non-food solid matrices. The preparation of such matrices are beyond the scope of this method (see Clause 12, Bibliography for further guidance).
NOTES:
1 Thermotolerant coliforms are also referred to as faecal coliforms although the organisms may not be of faecal origin.
2 A flow diagram of the procedure is shown in Appendix A.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	Standards Australia
ProductNote	Reconfirmed 27/07/2018. This standard has been reconfirmed in Australia in 2018 and remains current in New Zealand. Reconfirmation Notice 27/07/2018
Pages
ISBN
Committee	FT-020
Supersedes	DR 06120 AS 4276.6-1995 AS 4276.4-1995