Table of Contents

1 -  AS/NZS 4276.20:2003 WATER MICROBIOLOGY - EXAMINATION FOR COAGULASE POSITIVE STAPHYLOCOCCI, INCLUDING STAPHYLOCOCCUS...
1 -  PREFACE
1 -  METHOD
1 -  1 SCOPE
2 -  2 REFERENCED DOCUMENTS
2 -  3 PRINCIPLE
2 -  4 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
2 -  4.1 Culture media
2 -  4.1.1 Baird-Parker agar (BP)
2 -  4.1.2 Brain heart infusion (BHI) broth
2 -  4.1.3 Nutrient agar
2 -  4.2 Reagents (see AS 4276.2)
2 -  4.2.1 Saline solution, 0.85%
2 -  4.2.2 Rabbit blood plasma-
2 -  4.3 Reference cultures
2 -  4.3.1 Positive culture
2 -  4.3.2 Negative culture
2 -  4.3.3 Use of reference cultures
3 -  5 SAMPLES
3 -  5.1 Laboratory samples
3 -  5.2 Dilutions
3 -  6 PROCEDURE
3 -  6.1 Membrane filtration
3 -  6.2 Presumptive identification of coagulase positive staphylococci
3 -  6.3 Confirmation of coagulase positive staphylococci
5 -  6.4 Confirmation of Staphylococcus aureus
5 -  7 CALCULATION OF RESULT
6 -  8 TEST REPORT
6 -  9 BIBLIOGRAPHY
7 -  APPENDIX A - FLOW CHART FOR STAPHYLOCOCCI TESTING
8 -  APPENDIX B - CULTURE MEDIA
8 -  B1 SCOPE
8 -  B2 BAIRD-PARKER AGAR
8 -  B2.1 Preparation of complete medium
8 -  B2.2 Basal medium
9 -  B2.3 Concentrated egg yolk emulsion
9 -  B2.4 Solutions
9 -  B3 BRAIN HEART INFUSION BROTH
9 -  B3.1 Formulation
9 -  B3.2 Preparation
10 -  B3.3 Preparation of calf brain infusion
10 -  B3.4 Preparation of beef heart infusion

Abstract

RECONFIRMATION NOTICE
Technical Committee FT-020 has reviewed the content of this publication and in
accordance with Standards Australia procedures for reconfirmation, it has been determined
that the publication is still valid and does not require change.
Certain documents referenced in the publication may have been amended since the original
date of publication. Users are advised to ensure that they are using the latest versions of
such documents as appropriate, unless advised otherwise in this Reconfirmation Notice.
Approved for reconfirmation in accordance with Standards Australia procedures for
reconfirmation on 22 November 2013.
The following are represented on Technical Committee FT-020:
ACT Health
Australian Society for Microbiology
Australian Water Association
Department of Health Victoria
National Association of Testing Authorities, Australia
National Measurement Institute
NSW Forensic and Analytical Science Services
Path West Laboratory Medicine, WA
Plastics and Chemicals Industries Association
Queensland Health
Queensland University of Technology
University of Melbourne
Water Services Association of Australia

Scope

This Standard sets out a method for detecting coagulase positive staphylococci and Staphylococcus aureus (S.aureus) in water by the membrane filtration method.
NOTES:
1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.
2 The optimum range for membrane filter counts is 20-80 colonies. If the confirmed total count from one or more membranes is less than 20, then the derived sample count, which is an estimate of the true count in the sample (population parameter), is regarded as having a low precision. At the other extreme, when membranes containing greater than 80 colonies are counted, the counting error tends to be large. Again, the derived counts are regarded as having low precision. Derived counts of low precision should be indicated on reports by flagging them as ‘estimated’ or ‘approximate’.
3 A flow diagram of the procedure is shown in Appendix A.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	Standards Australia
ProductNote	This standard has been reconfirmed in Australia in 2013 and remains current in New Zealand. Reconfirmation Notice 05-12-2013
Pages
ISBN
Committee	FT-020
Supersedes	DR 01106