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BS EN ISO 21872-1 : 2017

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BS EN ISO 21872-1 : 2017

MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE DETERMINATION OF VIBRIO SPP. - PART 1: DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO PARAHAEMOLYTICUS, VIBRIO CHOLERAE AND VIBRIO VULNIFICUS (ISO 21872-1:2017

British Standards Institution

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Table of Contents

Foreword<br>Introduction<br>1 Scope<br>2 Normative references<br>3 Terms and definitions<br>4 Principle<br>5 Culture media and reagents<br>6 Equipment and consumables<br>7 Sampling<br>8 Preparation of the test sample<br>9 Procedure<br>10 Expression of results<br>11 Performance characteristics of the method<br>12 Test report<br>Annex A (normative) - Diagram of procedure<br>Annex B (normative) - Composition and preparation<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;of the culture media and reagents<br>Annex C (informative) - Conventional PCR for the<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;detection of Vibrio parahaemolyticus,<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;thermostable direct haemolysin (tdh) and<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;thermostable direct related haemolysin (trh)<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;genes, Vibrio cholerae and Vibrio vulnificus<br>Annex D (informative) - Real-time PCR for the<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;detection of Vibrio parahaemolyticus,<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;thermostable direct haemolysin gene (tdh)<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;and Vibrio vulnificus<br>Annex E (informative) - Results of an interlaboratory<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;study<br>Bibliography

Abstract

Defines a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract.

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Document Type Standard
Status Current
Publisher British Standards Institution
Committee AW/9
Supersedes
  • DD ISO/TS 21872-1 : 2007