Table of Contents

Foreword<br>Introduction<br>1 Scope<br>2 Normative references<br>3 Terms and definitions<br>4 Symbols and abbreviated terms<br>5 Materials<br>6 Technical equipment<br>7 Nanoparticle sample preparation<br>8 Preparations<br>9 Evaluation of nanoparticle impact on ROS generation in cells<br>10 Data analysis and results<br>Annex A (informative) - Alternate cell lines<br>Annex B (informative) - Alternate fluorescence characterization<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;techniques<br>Annex C (informative) - Suspension preparation and characterization<br>Annex D (informative) - Example experimental data from RAW 264.7<br>Bibliography

Abstract

Defines how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H[2]DCFDA assay.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	British Standards Institution
Pages
ISBN
Committee	NTI/1