Table of Contents

Foreword
Introduction
1 Scope
2 Terms and definitions
3 Micronucleus assay methodology used in this standard
4 Confidentiality of personal information
5 Laboratory safety requirements
6 Calibration source(s), calibration curve, and minimum
   resolvable dose
7 Responsibility of the customer
8 Responsibility of the CBMN laboratory
9 Accidental overexposure involving few individuals
10 Population triage
11 Quality assurance and quality control
Annex A (informative) - Sample data sheet for recording
        micronuclei in binucleated cells
Annex B (informative) - Automation of micronuclei scoring
Annex C (informative) - Instructions for customer (sample)
Annex D (informative) - Sample questionnaire
Annex E (informative) - Sample of report for single assessment
Annex F (informative) - Example group sample report
Bibliography

Abstract

Describes the following: a) confidentiality of personal information for the customer and the laboratory; b) laboratory safety requirements; c) radiation sources, dose rates, and ranges used for establishing the calibration reference dose-effect curves allowing the dose estimation from CBMN assay yields and the minimum resolvable dose; d) performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring; e) scoring criteria; f) conversion of micronucleus frequency in binucleated cells into an estimate of absorbed dose; g) reporting of results; h) quality assurance and quality control; and i) informative annexes containing examples of a questionnaire, instructions for customers, a microscope scoring data sheet, a sample report and advice on strengths and limitations of current automated systems for automated micronucleus scoring.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	International Organization for Standardization
Pages
ISBN
Committee	TC 85
Supersedes	ISO/DIS 17099 : 60.00 (2014)