M00023655
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NANOTECHNOLOGIES - IN VITRO MTS ASSAY FOR MEASURING THE CYTOTOXIC EFFECT OF NANOPARTICLES
International Organization for Standardization
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Availability date: 11/05/2021
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Materials
6 Apparatus
7 Nanoparticle test sample preparation
8 Preparations
9 Characterization of nanoparticle impact on cell viability
10 Cell viability analysis
11 Interpertation of Assay Results
Annex A (informative) - Potential cell lines and assays
Annex B (informative) - Example: the MTS assay using
the A549 cell line (EMPA-NIST protocol)
Annex C (informative) - Example: MTS assay using the
RAW 264.7 cell line (IANH protocol)
Bibliography
Describes a method for evaluating the effects of nano-objects and their aggregates and agglomerates (NOAA) on cellular viability using the MTS assay.
Published | |
Document Type | Standard |
Status | Current |
Publisher | International Organization for Standardization |
Pages | |
ISBN | |
Committee | TC 229 |