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ISO 19007 : 1ED 2018

M00023655

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ISO 19007 : 1ED 2018

NANOTECHNOLOGIES - IN VITRO MTS ASSAY FOR MEASURING THE CYTOTOXIC EFFECT OF NANOPARTICLES

International Organization for Standardization

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Table of Contents

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Materials
6 Apparatus
7 Nanoparticle test sample preparation
8 Preparations
9 Characterization of nanoparticle impact on cell viability
10 Cell viability analysis
11 Interpertation of Assay Results
Annex A (informative) - Potential cell lines and assays
Annex B (informative) - Example: the MTS assay using
        the A549 cell line (EMPA-NIST protocol)
Annex C (informative) - Example: MTS assay using the
        RAW 264.7 cell line (IANH protocol)
Bibliography

Abstract

Describes a method for evaluating the effects of nano-objects and their aggregates and agglomerates (NOAA) on cellular viability using the MTS assay.

General Product Information

Document Type Standard
Status Current
Publisher International Organization for Standardization
Committee TC 229