Table of Contents

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Interferences
5 Principle
6 Apparatus and materials
7 Reagents, cells and media
8 Sampling and samples
9 Procedure
10 Validity criteria
11 Assessment criteria
12 Test report
Annex A (informative) - Settings of the luminometer
Annex B (informative) - Plate setup
Annex C (informative) - Bioassay characteristics
        and details
Annex D (informative) - Test set up for chemicals
        and extracts
Annex E (informative) - Preparation of dilution
        series
Annex F (informative) - Validation data
Annex G (informative) - Statistical assessment
Annex H (informative) - Calculation of 17 Beta-estradiol
        equivalents
Annex I (informative) - Measurement of the lowest
        ineffective dilution (LID) of waste water - A simplified
        evaluation for testing of waste water
Bibliography

Abstract

Defines a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	International Organization for Standardization
Pages
ISBN
Committee	TC 147