Table of Contents

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation of reagents
        and buffers
Annex C (informative) - Real-time RT-PCR mastermixes and
        cycling parameters
Annex D (informative) - Real-time RT-PCR primers and
        hydrolysis probes for the detection of HAV, norovirus
        GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain MC[0]
        for use as a process control
Annex F (informative) - RNA extraction using the NucliSENS[R]
        system
Annex G (informative) - Generation of dsDNA control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies and
        performance characteristics
Bibliography

Abstract

Describes a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	International Organization for Standardization
Pages
ISBN
Committee	TC 34
Supersedes	ISO/DIS 15216-1 : 60.00 (2017)