Table of Contents

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents, culture media and reagents
6 Equipment
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Performance data
Annex A (normative) - Flow diagram of the
        screening procedure
Annex B (normative) - Flow diagram of the
        isolation and confirmation procedure
Annex C (informative) - Identification of Shiga
        toxin-producing Escherichia coli
        (STEC) by multiplex PCR amplification
        of virulence genes and detection of PCR
        products by agarose gel electrophoresis
Annex D (informative) - Internal amplification control
Annex E (informative) - Primers and probes for the
        PCR assays
Annex F (normative) - Isolation of STEC strains
Bibliography

Abstract

Specifies the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.

General Product Information

Published
Document Type	Standard
Status	Current
Publisher	International Organization for Standardization
Pages
ISBN
Committee	TC 34